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Establishing reference ranges and normal values for coagulation screening in healthy Indian male volunteers enrolled for a longitudinal study
Rehan Ahmed1, Prosenjit Ganguli2, Natwar Singh1, Surinderpal Singh3, Umesh Das Gupta4, Yogesh K Jaiswal5, Zahid Asharaf6, Prasanna Reddy7, Velu Nair8
1 Department of Clinical Hematology, Centre for Stem Cell Therapy and Research, Army Hospital (Research and Referral), New Delhi, India
2 Department of Pathology, Command Hospital (Eastern Command), Kolkata, West Bengal, India
3 Administration, DADMS (Deputy Additional Director of Medical Services), Tajganj, Agra, India
4 Ex-Director, Scientist G, National Jalma Institute of Leprosy and other Mycobacterial Diseases-ICMR (NJIL & OMD-ICMR), Tajganj, Agra, India
5 Faculty of School of Studies, Biochemistry, Jiwaji University, Gwalior, Madhya Pradesh, India
6 Department of Biotechnology, Jamia Milia Islamia University, New Delhi, India
7 Department of Cardiorespiratory, Defence Institute of Physiology & Allied Sciences, Defence Research & Development Organization, New Delhi, India
8 Ex-DGMS-Army (Director General Medical Services) Palam Vihar, Gurgaon, Haryana, India
Correspondence Address:
Prof. Prosenjit Ganguli
Department of Pathology, Command Hospital (Eastern Command), Kolkata, West Bengal
India
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/bbrj.bbrj_140_18
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Background: The study was designed for establishing reference ranges and normal values for coagulation screening in healthy Indian male volunteers. At present, there are no standard parameters established for coagulation screening assays of Indian population. The parameters used as a reference in the coagulation assays are of Western origin. We know that ethnicity of the western population is different from the Indian population which may result in a different set of reference ranges of the coagulation assay. It is necessary to determine the mean normal values for the coagulation assay, namely prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), and fibrinogen in an Indian population. The aim of the study was to establish the reference ranges of coagulation assays for all future experiments on the cohort. Methods: Six hundred healthy male volunteers were sampled and underwent coagulation testing at a seven hundred-bedded hospital at Jammu (Jammu). Fresh normal pool plasma (FNPP) was prepared concurrently and investigated (n = 50 volunteers). In the study population, the arithmetic means of the coagulation assay were as follows: PT: 13.00 s, APTT: 34 s, TT: 17.3 s, and fibrinogen: 298 mg/dl (19 s), and in the FNPP, it was 12.8 s, 33.2 s, 17 s, and 298 mg/dl (19 s), respectively. Results: The reference range of coagulation screening in our study was established as the following values: PT: 10.7–15.3 s, APTT: 26.8–41.2 s, TT: 12.8–21.7 s, and fibrinogen: 223–372 mg/dl. This study has set a reference range of normal values for coagulation assay screening in longitudinal studies where these tests were repeated on the same set of individuals at six-month interval for the subsequent 3 years. Conclusion: We see no effect of age on Coagulation cascade in our study. Overall mean values resembled with various age groups in coagulation cascade. These parameters of Coagulation cascade set a standard for high altitude studies where these tests are in normal procedure.
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